Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules 174346-82-8 | tert-Butyl 2-(hydroxymethyl)azetidine-1-carboxylate | MFCD03790936 | 1g
Ambeed | 3-Chlorothiobenzamide | 5g | 600842207 | A516790 | 2548-79-0 | MFCD00276993 | 171.640 | C7H6ClNS
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Seracare Life Sciences Inc 10X TRIS-GLYCINE TRANS BUFFER
10X Tris-glycine Transfer Buffer is intended for Western blotting and gel electrophoresis. It is ideal for preparing a standard Western blot transfer buffer (Towbin) and for use as a gel electrophoresis buffer for native Tris-glycine gel without SDS. pH=8.3. Conductivity controlled.
Research Products International Corp Potassium Phosphate, Monobasic, ACS Grade, 10 KG
Potassium Phosphate, Monobasic, ACS Grade is a chemical compound that holds significance in the life science research, particularly in molecular biology, cell culture, and biochemistry fields.
Teknova 20x PBS with 0.05% Tween-20. 500mL, Sterile. 8 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Biotang Inc Phosphate-Buffered Saline PBS, 10X, pH 7.6, 1000 ml
Phosphate-Buffered Saline PBS, 10X, pH 7.6, 1000 ml.
![Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 100 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661398.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 100 Tablets per Bottle
PBS [Phosphate Buffered Saline] 200mL Tablets are solid tablets designed for easy dissolution in water, making it simple to prepare 200mL of phosphate-buffered saline. These tablets are ideal for laboratories that frequently need to prepare large volumes of PBS for use in cell culture, microbiology, and molecular biology research. The tablets dissolve quickly and efficiently, ensuring that the resulting solution maintains the proper pH and osmolarity for reliable experimental results. Each bottle contains 100 tablets, providing an economical and convenient way to prepare PBS without the need for precise measuring or mixing of individual components.
Apexbio Technology LLC Annexin V-FITC/7-AAD Apoptosis Kit 20assays
Annexin V is a calcium-dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) Normally PS is localized to the inner leaflet of the cell membrane lipid bilayer but during early stages of apoptosis PS flips from the inner leaflet to the outer surface of the plasma membrane where it is exposed to the extracellular environment Annexin V displays a high affinity for PS Fluorescein (FITC)-conjugated Annexin V can be used as a fluorescent probe in flow cytometry or fluorescent microscopy to detect apoptosis within 10-20 minutes The nuclear dye 7-Aminoactinomycin D (7-AAD) specifically binds to double-stranded DNA and fluoresces upon binding Under normal circumstances 7-AAD is cell-impermeable however in late apoptotic and dead cells 7-AAD can pass through cell membranes staining DNA Combining Annexin V-FITC with 7-AAD staining cells at different stages of apoptosis and dead cells can be distinguished effectively
Boston Bioproducts Inc Sodium Phosphate Buffer (1 M, pH 7.2) - 50ml
Sodium Phosphate Buffer (1 M pH 7 2) - 50ml (For Research Use and Further Manufacturing Only)