Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific Tris Acetate Ultra Pure, 1 Kg
Tris Acetate Ultra Pure is a high-purity buffering agent used in a variety of biochemical applications. This reagent is ideal for high-precision applications such as electrophoresis, protein analysis, and other laboratory techniques requiring a stable pH. The ultra-pure grade ensures minimal interference with sensitive experiments, maintaining the integrity of reactions. This 1kg bottle is perfect for large-scale buffer preparations, providing consistent buffering power across a range of experiments. Tris acetate is commonly used in molecular biology, immunology, and protein chemistry, particularly for its ability to maintain a stable pH in high-purity systems.
Bioworld Tricine-SDS Sample Buffer, Non-Reducing (2X), 50 mL
Product DescriptionTricine-SDS is a sample buffer under non-reducing conditions for protein analysis. It has a concentration of 2X and contains 900 mM Tris-HCl with a pH of 8.45, 8% SDS, 20% glycerol, 0.01% Coomassie blue, and 0.01% phenol red.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Teknova 1M Tris-HCl, pH 10.2. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Selleck Chemical LLC P62-mediated mitophagy inducer
P62-mediated mitophagy inducer (PMI) is a P62-mediated activator of mitophagy that functions independently of parkin recruitment or mitochondrial membrane potential ( m) collapse It remains effective even in cells lacking a fully functional PINK1/Parkin pathway PMI stabilizes Nrf2 and enhances p62 expression thereby activating mitophagy
Biotang Inc E. Coli Lysis Buffer (Tris-HCl 50 mM,1% Triton,EDTA 1 mM, Beta-mercaptoethanol 10 mM), 250 ml
E. Coli Lysis Buffer (Tris-HCl 50 mM,1% Triton,EDTA 1 mM, Beta-mercaptoethanol 10 mM), 250 ml.
Ethos Biosciences Dilurex 1M Tris-HCL Buffer, 100ml, Ethos
Dilurex 1M Tris-HCL 10X Buffer is a classic laboratory buffer at pH 7.4. It does not include a saline ionic component and is free to be mixed with other components as needed in your laboratory. kip the tedium of weighing powders and stocking raw materials that can expire. Our buffer is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences buffers for their proven track record in top-tier publications and protocols - upgrade your productivity today.
![Alkali Scientific TG 10X Solution [TRIS Glycine Buffer 10X Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661625.jpg-250.jpg)
Alkali Scientific TG 10X Solution [TRIS Glycine Buffer 10X Solution], 1 Liter
TRIS Glycine Buffer 10X Solution is a high-performance buffer designed for use in electrophoresis, particularly for running SDS-PAGE gels. The 1-liter bottle of 10X solution allows for efficient dilution and preparation of multiple electrophoresis runs. This buffer system is well-suited for separating a wide range of proteins, providing reliable and consistent results. The TRIS-Glycine formulation is known for its excellent buffering capacity and is widely used in molecular biology and biochemistry for protein analysis. This buffer is compatible with numerous protein electrophoresis protocols, including those for high-precision applications such as Western blotting and protein quantification.
Nusep Inc NUVIEW UNIV GEL 10 10-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 10% 10well. Cassette size: 10 x 8 cm. Fits Hoefer, Cytiva, Merck Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.